What is the Lineweaver Burk equation?
The Lineweaver-Burk equation is a linear equation, where 1/V is a linear function of 1/[S] instead of V being a rational function of [S]. The Lineweaver-Burk equation can be readily represented graphically to determine the values of Km and Vmax. Given a Lineweaver-Burk plot, determine the Vmax of a particular enzyme.
How do you calculate Vmax from Lineweaver Burk plot?
So, knowing the initial rate, Vo, and the various concentration of the substrate, you can create a straight line. The line plot represents the slope of Km/Vmax and y-intercept of 1/Vmax. Next, use the reciprocal of the y-intercept to calculate the Vmax of the enzyme activity.
How do you plot a Lineweaver Burk plot?
For a Lineweaver-Burk, the manipulation is using the reciprocal of the values of both the velocity and the substrate concentration. The inverted values are then plotted on a graph as 1/V vs. 1/[S]. Because of these inversions, Lineweaver-Burk plots are commonly referred to as ‘double-reciprocal’ plots.
What is km in Lineweaver Burk plot?
Lineweaver–Burk Plot. where V is the reaction velocity (the reaction rate), Km is the Michaelis–Menten constant, Vmax is the maximum reaction velocity, and [S] is the substrate concentration.
Why do we use Lineweaver Burk plot?
The Lineweaver–Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax, before the wide availability of powerful computers and non-linear regression software. It also gives a quick, visual impression of the different forms of enzyme inhibition.
What is Vmax in Michaelis Menten equation?
The Michaelis-Menten equation for this system is: Here, Vmax represents the maximum velocity achieved by the system, at maximum (saturating) substrate concentrations. KM (the Michaelis constant; sometimes represented as KS instead) is the substrate concentration at which the reaction velocity is 50% of the Vmax.
How do you calculate Km and Vmax on a graph?
From the graph find the maximum velocity and half it i.e. Vmax/2. Draw a horizontal line from this point till you find the point on the graph that corresponds to it and read off the substrate concentration at that point. This will give the value of Km.
What units is Vmax measured in?
Vmax “represents the maximum rate achieved by the system, at maximum (saturating) substrate concentrations” (wikipedia). Unit: umol/min (or mol/s).
How do you plot a graph Michaelis Menten?
Plotting the Michaelis-Menten Curve Label the y ax- sec/micro-mole of V or velocity of reaction. Insert different values of [S] into the Michaelis-Menten equation, along with the values found for Km and Vmax, to solve for V. Plot the values for [S] on the x-axis and the corresponding solved values for V on the y-axis.
How do you solve for Vmax and Km?
[S] / v = Km / Vmax + [S] / Vmaxy intercept = Km / Vmax.gradient = 1 / Vmax.x intercept = -Km.
How do you calculate Vmax?
You can estimate KM and Vmax from the graph of initial velocity versus [S].Run a series of reactions with constant [Etot], varying [S], and measure Vo.Graph Vo vs. [S].Estimate Vmax from asymptote.Calculate Vmax/2.read KM from graph.